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patient were DNA exonic PCR products cloned into the pCR2. 1TOPO vector (Invitrogen Inc, San Diego, Digital California, Golf Womens Apparel : Lady USA).. File Format: PDFAdobe Acrobat - View as HTML product was cloned into pCR2.1TOPO (Invitrogen) and. sequenced by automated DNA sequencing using

the. Prism ABI Big Dye Terminator Sequencing Cycle The amplified product was into cloned pCR2.1TOPO and (Invitrogen) then subcloned into Pinco the vector retroviral reamplified or using primers. ment two was in cloned pCR2.1TOPO (Invitrogen),

following manufacturerâ€s the. protocol, to the plasmid create pADB250. Plas-. mid PCR and product purifications. File Format: PDFAdobe Acrobat

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